Spruce - Complete cDNA libraries description

NAME:

Male strobili developmental sequence

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Tree 13-271

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Expanding male strobili

TISSUE:

Entire strobilus

STAGE:

Three stages of preformed male strobili at end of winter dormancy were pooled: swollen fully closed buds, partly open buds and fully open buds

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Female cones developmental sequence

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Tree 13-271

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Expanding female cones

TISSUE:

Entire cone

STAGE:

Three stages of preformed female cones at end of winter dormancy were pooled: swollen fully closed buds, partly open buds and fully open buds

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Vegetative buds developmental sequence

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Tree 13-271

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Expanding whole vegatative buds

TISSUE:

Needles and primary shoot

STAGE:

Three stages of preformed vegetative buds at end of winter dormancy were pooled: swollen fully closed buds, partly open buds and fully open buds

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Non-lignified secondary xylem from mature trees

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Two trees of provenance 5333 and one from 5206

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Stem from ground to lower part of live crown, on 33 year old tree

TISSUE:

Non-lignified differentiating secondary xylem from normal vertical trees.

STAGE:

Non-lignified secondary xylem from trees harvested 2.5 hours, 6 hours and 11 hours after day break. Tissue was harvested in mid-June, during formation of early wood.

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Cambium and phloem from mature trees

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Two trees of provenance 5333 and one from 5208

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Stem from ground to lower part of live crown, on 33 year old tree

TISSUE:

Cambium and phloem region from normal vertical trees

STAGE:

Cambium and phloem tissue scraped from inside of bark from trees harvested 2.5 hours, 6 hours and 11 hours after day break were pooled. Tissue was harvested in mid-June, during formation of early wood.

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Secondary xylem of girdled saplings

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Main stem of 4 year old saplings, approximately 60 cm tall

TISSUE:

Secondary xylem tissues of trees girdled by removing a ring of bark ca. 1 cm wide at the midpoint of the main stem. Tissues were pooled from above and below the girdle.

STAGE:

Secondary xylem at all stages of differentiation from both current and previous seasons' growth, from trees harvested 1 and 7 days after girdling treatment.

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Cambium, phloem and bark of girdled saplings

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Main stem of 4 year old saplings, approximately 60 cm tall

TISSUE:

Vascular cambium, secondary phloem and bark of trees girdled by removing a ring of bark ca. 1 cm wide from the midpoint of the main stem. Tissues were pooled from above and below the girdle.

STAGE:

Vascular cambium, secondary phloem, and bark from trees harvested 1 and 7 days after girdling treatment

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Elongating ROOTS tips - saplings

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

Roots from 60 cm tall seedlings.

TISSUE:

Tender roots tips free of michorizee

STAGE:

Activelyl elongating roots

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

Tender root tips free of michorizee

NAME:

Primary and secondary SHOOT -N fertilization treatments

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

Main stem region producing secondary growth on 60 cm tall seedlings

TISSUE:

Xylem, pith, cambium, phloem, bark

STAGE:

Primary & secondary shoot, secondary phloem pooled from plants fertilized with low and high NH4NO3

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Immature somatic EMBRYOS

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

Whole intact somatic embryos comprised comprised of senescing suspensor and bullet stage

TISSUE:

Whole embryo

STAGE:

Collected after two-weeks on maturation medium containing ABA

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Clean ROOTS systems - N treatments

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

whole root systems form 15 cm tall seedlings

TISSUE:

Whole roots systems, predominantly free of michorizee

STAGE:

Roots from plants fertilized with low and high NH4NO4

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Clean ROOTS systems - P treatments

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

whole root systems form 15 cm tall, 4 month old seedlings produced by somatic embryogenesis

TISSUE:

Whole roots systems, predominantly free of michorizee

STAGE:

Tissues were pooled from several plants receiving daily fertilization varying potasium levels (either 0.02 mM or 2 mM PO4), collected at 4 hours, 24 hours, 2 days, 4 days or 7 days of treatment

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Clean ROOTS systems - Diurnal cycle

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

whole root systems form 15 cm tall, 4 month old seedlings produced by somatic embryogenesis

TISSUE:

Whole roots systems, predominantly free of michorizee

STAGE:

Tissues were pooled from several plants grown under 20h light/4h dark and collected every 2 hours over a period of 28 hours (4 dark time points and 11 time points in light).

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

ROOT XYLEM - mature trees

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Three trees Unknown

SEX:

Hermaphrodite

ORGAN:

Roots from 9 year old trees measuring approximately 4.5 m tall, and 10 cm in diameter.

TISSUE:

Differentiating xylem from roots 1 cm in diameter or larger

STAGE:

Non-lignified xylem tissues from larger roots in early part of growing season (June).

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Annual flush SHOOTS diurnal cylce- trees

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Several trees unknown

SEX:

Hermaphrodite

ORGAN:

Stem annual flush from top of 10 year old trees, approximately 5 m tall and 12 cm in diameter

TISSUE:

Pith, xylem, cambium, phloem and bark. Needles were removed.

STAGE:

Shoot comprised of primary and secondary growth (no needles) of annual flush pooled from several trees collected over a natural 15h40min light / 9h20min dark cycle every three hours

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Mature Somatic EMBRYOS

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

Whole intact somatic embryos comprised of cotyledonary, root, and apical primordia and hypocotyl

TISSUE:

Whole embryo

STAGE:

Mature embryo obtained by growing immature somatic embryonic massess on media containing ABA for 8 weeks

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation