Poplar - Complete cDNA libraries

NAME:

Secondary STEMS - N fertilization, stem girdling treatments

AUTHORS :

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Boyle, B; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Populus trichocarpa x P. deltoides

STRAIN:

H11-11

SEX:

MALE

ORGAN:

Whole secondary stems of trees approximately 80-120 cm tall

TISSUE:

Whole stems with secondary growth, composed of secondary xylem, secondary phloem, cambium, and bark

STAGE:

Stems harvested from the midsection of trees subjected either to fertilization with 0 or 25 mM ammonium nitrate for 1, 3, or 7 days, or removal of a ring of bark from the main stem (stem girdling) for 1 or 6 days.

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Primary STEMS - N fertilization, metabolite uptake treatments

AUTHORS :

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Boyle, B; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Populus trichocarpa x P. deltoides

STRAIN:

H11-11

SEX:

MALE

ORGAN:

Whole primary stems of trees approximately 80-120 cm tall

TISSUE:

Primary xylem and primary phloem from whole stems.

STAGE:

Wholes stems with primary growth were harvested between LPI 1 and 4 from trees treated either with low vs. high levels of ammonium nitrate fertilization for 7 days, or with different sources of nitrogen or carbon for 4 or 24 hours.

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

LEAVES - N fertilization, metabolite uptake treatments

AUTHORS :

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Boyle, B; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Populus trichocarpa x P. deltoides

STRAIN:

H11-11

SEX:

MALE

ORGAN:

Leaves of trees approximately 80-120 cm tall

TISSUE:

Leaves

STAGE:

Young leaves (LPI 1-3), mature leaves (LPI 7-9) and senescing leaves (LPI 15-17) from trees treated either with low vs. high levels of ammonium nitrate fertilization for 7 days, or with different sources of nitrogen or carbon for 4 or 24 hours.

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

SHOOT TIPS - N fertilization, stem girdling, metabolite uptake treatments

AUTHORS :

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Boyle, B; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Populus trichocarpa x P. deltoides

STRAIN:

Shoot tips of trees approximately 80-120 cm tall

SEX:

MALE

ORGAN:

Shoot apex, primary stem and developing leaves up to and including LPI 1

TISSUE:

Actively growing shoot tips of trees subjected to fertilization with 0 or 25 mM ammonium nitrate for 1, 3, or 7 days, or removal of a ring of bark from the main stem (stem girdling) for 1 or 6 days, or treatment with different sources of nitrogen and carbon for 4 or 24 hours.

STAGE:

Populus trichocarpa x P. deltoides

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

SHOOT TIPS and SECONDARY STEMS - Diurnal sequence

AUTHORS :

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Boyle, B; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

P. trichocarpa x deltoides

STRAIN:

H11-11

SEX:

MALE

ORGAN:

Shoot tips and secondary stems of trees approximately 80 cm tall

TISSUE:

Shoot tips (including apex, primary stem and developing leaves up to and including LPI 1) and whole stems (wood and bark) undergoing secondary growth taken from between LPI 8 and LPI 15.

STAGE:

Pooled sample of tissues harvested every two hours during one 12 hour light/12 hour dark cycle, beginning 1 hour before the beginning of the light period and ending 3 hours after the end of the light cycle.

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

NAME:

Secondary XYLEM lignified and unlignified, secondary developing PHLOEM - Mature trees

AUTHORS :

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Boyle, B; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

P. trichocarpa x deltoides

STRAIN:

Beaupre 3226

SEX:

Female

ORGAN:

Current year's growth taken from two regions of mature tree trunk

TISSUE:

Secondary xylem, secondary phloem representing current year's growth of main stem of mature tree

STAGE:

Secondary developing lignified and unlignified xylem, secondary developing phloem

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation