EST Sequencing in Poplar
Summary
We produced 25 different cDNA libraries, and sequenced random clones in spruce (nearly 50 K) and poplar (14 K). Sequences have been analyzed and released; a sequence database has been developed and implemented.
View activity goals by clicking here.
cDNA Poplar Libraries
| Library code | Name | Quality sequences * | Data release | |
|---|---|---|---|---|
Quality reads | Average length | |||
Secondary STEMS, plants with altered N status: | 1299 | 486 | ||
Primary STEMS, plants with altered N status: | 2611 | 659 | ||
LEAVES, plants with altered N status: | 688 | 355 | ||
SHOOT TIPS, plants with altered N status: | 1265 | 429 | ||
GQ014 | Infected LEAVES - 8-day Melampsora medusae infection ( B. Boyle) | 1316 | 364 | October 2004 |
SHOOT TIPS AND SECONDARY STEMS - Diurnal sequence | 1648 | 750 | ||
GQ023 | Infected LEAVES with M. medusae 1, 2, and 4 days (B. Boyle) | 1368 | 541 | October 2004 |
XYLEM lignifed and unlignified, | 1396 | 520 | ||
| TOTAL QUALITY READS - POPLAR | 11591 |
|
|
* Quality sequences contain 100 nucleotides or more of Phred 20 quality, after vector sequence is removed.
Sequencing results in POPLAR
A total of 8 907 processed EST sequences containing a minimum of 100bp meeting a quality score of phred 20 or higher have been obtained from libraries GQ009, GQ010, GQ011, GQ012, GQ015, GQ024.
Sequences, sequence assemblies and blast reports may be visualized and searched in Biodata web pages hosted at the Center for Computational Genomics and Bioinformatics :
Order cDNA clones
You can order cDNA clones from our project.
Requests are filled free of charge or on cost recovery basis, depending on volume of the request, the type of institution (for-profit or non-for-profit) and the origin of the request (Canada or outside Canada).
For more information, click here : Order cDNA clones
