Activation-Tagging (poplar)
Activation-Tagging in poplar
This activity has been part of the first phase of Arborea (2002-2006). The research was led by Dr. Sharon Regan at Carleton University in Ottawa.
Our objective was to produce a large-scale population of poplar through activation tagging. This population will permit us to discover through gain-of-function mutagenesis, genes that have a putative role in vascular development or defense response. Our milestones were:
- Large-scale production of activation tagged lines in controlled greenhouses
- Screen lines for phenotypes related to vascular development and defense response
- Detailed phenotypic analysis, and isolation of the activated genes
- Long term growth and analysis of the activation tagged lines