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Description complète des banques d'ADNc d'épinette

La description des banques est présentement disponible en anglais seulement.



GQ001 - Male strobili developmental sequence

NAME:

Male strobili developmental sequence

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Tree 13-271

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Expanding male strobili

TISSUE:

Entire strobilus

STAGE:

Three stages of preformed male strobili at end of winter dormancy were pooled: swollen fully closed buds, partly open buds and fully open buds

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ002 - Female cones developmental sequence

NAME:

Female cones developmental sequence

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Tree 13-271

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Expanding female cones

TISSUE:

Entire cone

STAGE:

Three stages of preformed female cones at end of winter dormancy were pooled: swollen fully closed buds, partly open buds and fully open buds

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ003 - Vegetative buds developmental sequence

NAME:

Vegetative buds developmental sequence

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Tree 13-271

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Expanding whole vegatative buds

TISSUE:

Needles and primary shoot

STAGE:

Three stages of preformed vegetative buds at end of winter dormancy were pooled: swollen fully closed buds, partly open buds and fully open buds

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ004 - Non-lignified secondary xylem from mature trees

NAME:

Non-lignified secondary xylem from mature trees

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Two trees of provenance 5333 and one from 5206

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Stem from ground to lower part of live crown, on 33 year old tree

TISSUE:

Non-lignified differentiating secondary xylem from normal vertical trees.

STAGE:

Non-lignified secondary xylem from trees harvested 2.5 hours, 6 hours and 11 hours after day break. Tissue was harvested in mid-June, during formation of early wood.

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ006 - Cambium and phloem from mature trees

NAME:

Cambium and phloem from mature trees

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Two trees of provenance 5333 and one from 5208

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Stem from ground to lower part of live crown, on 33 year old tree

TISSUE:

Cambium and phloem region from normal vertical trees

STAGE:

Cambium and phloem tissue scraped from inside of bark from trees harvested 2.5 hours, 6 hours and 11 hours after day break were pooled. Tissue was harvested in mid-June, during formation of early wood.

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ007 - Secondary xylem of girdled saplings

NAME:

Secondary xylem of girdled saplings

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Main stem of 4 year old saplings, approximately 60 cm tall

TISSUE:

Secondary xylem tissues of trees girdled by removing a ring of bark ca. 1 cm wide at the midpoint of the main stem. Tissues were pooled from above and below the girdle.

STAGE:

Secondary xylem at all stages of differentiation from both current and previous seasons' growth, from trees harvested 1 and 7 days after girdling treatment.

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ008 - Cambium, phloem and bark of girdled saplings

NAME:

Cambium, phloem and bark of girdled saplings

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Main stem of 4 year old saplings, approximately 60 cm tall

TISSUE:

Vascular cambium, secondary phloem and bark of trees girdled by removing a ring of bark ca. 1 cm wide from the midpoint of the main stem. Tissues were pooled from above and below the girdle.

STAGE:

Vascular cambium, secondary phloem, and bark from trees harvested 1 and 7 days after girdling treatment

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ013 - Elongating ROOTS tips - saplings

NAME:

Elongating ROOTS tips - saplings

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

Roots from 60 cm tall seedlings.

TISSUE:

Tender roots tips free of michorizee

STAGE:

Activelyl elongating roots

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

Tender root tips free of michorizee

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GQ016 – Primary and secondary SHOOT -N fertilization treatments

NAME:

Primary and secondary SHOOT -N fertilization treatments

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

Main stem region producing secondary growth on 60 cm tall seedlings

TISSUE:

Xylem, pith, cambium, phloem, bark

STAGE:

Primary & secondary shoot, secondary phloem pooled from plants fertilized with low and high NH4NO3

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ017 - Immature somatic EMBRYOS

NAME:

Immature somatic EMBRYOS

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

Whole intact somatic embryos comprised comprised of senescing suspensor and bullet stage

TISSUE:

Whole embryo

STAGE:

Collected after two-weeks on maturation medium containing ABA

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ018 - Clean ROOTS systems - N treatments

NAME:

Clean ROOTS systems - N treatments

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

whole root systems form 15 cm tall seedlings

TISSUE:

Whole roots systems, predominantly free of michorizee

STAGE:

Roots from plants fertilized with low and high NH4NO4

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ019 - Clean ROOTS systems - P treatments

NAME:

Clean ROOTS systems - P treatments

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

whole root systems form 15 cm tall, 4 month old seedlings produced by somatic embryogenesis

TISSUE:

Whole roots systems, predominantly free of michorizee

STAGE:

Tissues were pooled from several plants receiving daily fertilization varying potasium levels (either 0.02 mM or 2 mM PO4), collected at 4 hours, 24 hours, 2 days, 4 days or 7 days of treatment

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ020 - Clean ROOTS systems - Diurnal cycle

NAME:

Clean ROOTS systems - Diurnal cycle

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

whole root systems form 15 cm tall, 4 month old seedlings produced by somatic embryogenesis

TISSUE:

Whole roots systems, predominantly free of michorizee

STAGE:

Tissues were pooled from several plants grown under 20h light/4h dark and collected every 2 hours over a period of 28 hours (4 dark time points and 11 time points in light).

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ022 - ROOT XYLEM - mature trees

NAME:

ROOT XYLEM - mature trees

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Three trees Unknown

SEX:

Hermaphrodite

ORGAN:

Roots from 9 year old trees measuring approximately 4.5 m tall, and 10 cm in diameter.

TISSUE:

Differentiating xylem from roots 1 cm in diameter or larger

STAGE:

Non-lignified xylem tissues from larger roots in early part of growing season (June).

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ025 - Annual flush SHOOTS diurnal cylce- trees

NAME:

Annual flush SHOOTS diurnal cylce- trees

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Several trees unknown

SEX:

Hermaphrodite

ORGAN:

Stem annual flush from top of 10 year old trees, approximately 5 m tall and 12 cm in diameter

TISSUE:

Pith, xylem, cambium, phloem and bark. Needles were removed.

STAGE:

Shoot comprised of primary and secondary growth (no needles) of annual flush pooled from several trees collected over a natural 15h40min light / 9h20min dark cycle every three hours

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ027 - Mature Somatic EMBRYOS

NAME:

Mature Somatic EMBRYOS

AUTHORS:

Morency, M.-J.; Cooke, J.; Pavy, N.; Parsons, L; Paule, C.; Séguin, A.; Retzel, E.; Butterfield, Y.; Barber, S.; Yang, G.; Stott, J., Siddiqui, A.; Holt, R., Marra, M.; MacKay, J.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

pg-653

SEX:

Hermaphrodite

ORGAN:

Whole intact somatic embryos comprised of cotyledonary, root, and apical primordia and hypocotyl

TISSUE:

Whole embryo

STAGE:

Mature embryo obtained by growing immature somatic embryonic massess on media containing ABA for 8 weeks

HOST

E. coli DH10B cells

VECTOR:

pBluescript II SK (+) XR Plasmid

RESTRICTION SITES:

Eco-RI, Xho-I

DESCRIPTION:

cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

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GQ028 - Cambium / phloem scrappings (Normalized library )

NAME:

Cambium / phloem scrappings (Normalized library )

AUTHORS:

Jacobi, V.  et al.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

14 year old plantation of Picea glauca (12 years + 2 year old seedlings)- private plantation of M.Réjean Julien

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Stem from 14 year old trees

TISSUE:

Cambium and phloem region from normal vertical trees

STAGE:

Pool of trees sampled at three different daylight times (early, mid-day, late) at three different seasonal points (june, july, august).

HOST

Xl1-Blue

VECTOR:

pAL17.3

RESTRICTION SITES:

Sfi1-A, Sfi1-B

DESCRIPTION:

Library synthesis was performed by Evrogen using methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32, e37. Starting material is 0,3 ug of total RNA.

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GQ029 - Xylem scrapings - AT NITE

NAME:

Xylem scrapings - AT NITE

AUTHORS:

Jacobi, V.  et al.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

10 year old Picea glauca trees grown in the field (plantation)

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Stem from 10 year old trees

TISSUE:

Xylem scrapings from normal vertical trees

STAGE:

Pool of trees sampled at three different times (22H00, 1H00, 4H00) during the night

HOST

Xl1-Blue

VECTOR:

pAL17.3

RESTRICTION SITES:

Sfi1-A, Sfi1-B

DESCRIPTION:

Library synthesis was performed by Evrogen using methodology described in Zhu et al. (2001) Biotechniques 30: 892-897. Starting material is 0,3 ug of total RNA

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GQ030 - Xylem planings - daytime and seasonal

NAME:

Xylem planings - daytime and seasonal

AUTHORS:

Jacobi, V.  et al.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

14 year old plantation of Picea glauca (12 years + 2 year old seedlings)- private plantation of M.Réjean Julien

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Stem from 14 year old trees

TISSUE:

Xylem planings from normal vertical trees

STAGE:

Pool of trees sampled at three different times (early, mid-day, late) at three different seasonal points (june, july, august). 

HOST

Xl1-Blue

VECTOR:

pAL17.3

RESTRICTION SITES:

Sfi1-A, Sfi1-B

DESCRIPTION:

Library synthesis was performed by Evrogen using methodology described in Zhu et al. (2001) Biotechniques 30: 892-897. Starting material is 0,3 ug of total RNA

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GQ031 - Xylem Scrapings (Normalized library)

NAME:

Xylem Scrapings (Normalized library)

AUTHORS:

Jacobi, V.  et al.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Wood property progeny test (Réserve de Mastigouche, CFS Jean Beaulieu). 1 tree from each of 40 families. Approx 27 years of age. Trees were pooled at time of collection. Collected on July 20 pm -21 am.

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Stem from 27 year old trees

TISSUE:

Xylem scrapings 2 m above ground level, one log approx.50 cm in length. All trees were pooled 3-4 trees per 50 mL tube. 

STAGE:

Collected on July 20 pm -21 am. 

HOST

Xl1-Blue

VECTOR:

pAL17.3

RESTRICTION SITES:

Sfi1-A, Sfi1-B

DESCRIPTION:

Library synthesis was performed by Evrogen using methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32, e37. Starting material is 0,3 ug of total RNA. 

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GQ032 - Shoot tip (Normalized library)

NAME:

Shoot tip (Normalized library)

AUTHORS:

Jacobi, V.  et al.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

QTL population large seedlings, Family 25-16 (or 28-56). Rooted cuttings, rooted in 2002. Destructive sampling. Size of plants 30+ cm.

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Terminal leader and branches with needles (pooled from whole plant) 

TISSUE:

Shoot tip collected from terminal leader and from branches with needles (pooled from whole plant) 

STAGE:

Collected weekly, from 1st week in July to 30 October, 2005. 2 seedlings per collection date 

HOST

Xl1-Blue

VECTOR:

pAL17.3

RESTRICTION SITES:

Sfi1-A, Sfi1-B

DESCRIPTION:

Library synthesis was performed by Evrogen using methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32, e37. Starting material is 0,3 ug of total RNA.

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GQ033 - Terminal leader (Normalized library)

NAME:

Terminal leader (Normalized library)

AUTHORS:

Jacobi, V.  et al.

ORGANISM:

Picea Glauca (Moench.) Voss

STRAIN:

Wood property progeny test (Réserve de Mastigouche, CFS Jean Beaulieu). 1 tree from each of 40 families. Approx 27 years of age. Trees were pooled at time of collection. Collected on July 20 pm -21 am. SLIMS

SEX:

Hermaphrodite (Monoecious)

ORGAN:

Terminal leader 

TISSUE:

Terminal leader, without neeldes, terminal bud and sub-terminal buds. Stem piece 2-3 cm in length (includes bark).

STAGE:

Collected on July 20 pm -21 am.

HOST

Xl1-Blue

VECTOR:

pAL17.3

RESTRICTION SITES:

Sfi1-A, Sfi1-B

DESCRIPTION:

Library synthesis was performed by Evrogen using methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32, e37. Starting material is 0,3 ug of total RNA.

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